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Margate have sacked boss Terry Brown after losing 3-0 at relegation rivals St Albans on Saturday.
Only goal difference keeps Margate out of the Vanarama National League South relegation zone and Brown's job is rumoured to have been on the line recently.
Margate's patience ran out after losing at St Albans, who themselves parted company with their manager prior to the game. St Albans are now within two points of Margate in the league table.
A statement from Margate FC said: "The club would like to place on records it thanks for all their hard work over the past three seasons which saw them achieve promotion last year by winning the Ryman Premier League Play-offs beating Hendon 1-0 in May.
"MFC would like to wish Terry and Stuart all the best in their future endeavours.
"A further statement will follow in due course."
Margate have picked up just 18 points from 19 league games, winning just four matches.
After losing in the FA Trophy at Ryman League Dulwich last week, Margate's next game is not until December 19 when they host Gosport.