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Kent's Joe Denly faces a race against time to be fit for England's Test series against New Zealand later this month.
Denly has been ruled out for the rest of the T20 series after turning his ankle in training prior to the first T20 international, which took place on Friday.
The ECB have now confirmed that Denly has sustained ligament damage to his right ankle.
A statement confirmed: "He will now start a rehabilitation programme and will be routinely assessed ahead of the two-match Test series against the Black Caps starting on November 21."
England will not call up a replacement to the squad for the remaining four games.
Kent skipper Sam Billings made a return to wicketkeeping in the first game, which England won by seven wickets with nine balls to spare.
Billings finished 14 not out from 11 balls, putting on an unbroken 32 with captain Eoin Morgan to guide the tourists to victory.