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Kenny Clark and Tyrone Sterling have left Dartford after failing to agree terms for next season.
The defenders, who both joined the club last summer, were offered new deals by Darts manager Tony Burman.
But Burman said: "I feel I have given both players enough time to make their decisions and now is the time for me to move forward with my plans for next season."
Both players made 31 starts for Dartford last season as Burman's side were relegated from Skrill Premier.
Meanwhile, defender Mat Mitchel-King, midfielder Alex Woodyard and strike Mikel Suarez have all been invited back for pre-season.