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The FA have told Ebbsfleet they must replay their game at Concord Rangers in full on Thursday.
Ebbsfleet appealed against the original ruling by the Football Conference after floodlight failure at the Impact Arena forced play to be abandoned last Tuesday.
Goals from Ben May and Daryl McMahon had the Fleet 2-0 in front but Michael Corcoran had just been sent-off when the lights went out, seven minutes into the second half.
Ebbsfleet boss Steve Brown criticised the "antiquated" FA rule which says abandoned games must be replayed in full - unlike in some European countries - but the club's appeal on Wednesday was unsuccessful.
Brown's side can qualify for the Skrill South play-offs with a win at Concord as they're two points ahead of sixth-placed Dover Athletic with a game in hand. Admission will be free for the replayed game.
The regular season finishes on Saturday, with Ebbsfleet at home to Dorchester. Supporters can also get into that game, at Stonebridge Road, for free.