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Manager Andy Hessenthaler may have to break up his new-look defence when Gillingham host Rotherham on Tuesday night.
Hessenthaler put together a completely different back four for the trip to Port Vale on Saturday but there are doubts whether Simon King can last another 90 minutes so soon.
His appearance at the weekend was his first full game since early October, when he was on loan at Plymouth.
Hessenthaler said: "King’s situation is something we will have to address in the next few days.
"Can it (his ankle) sustain Saturday, Tuesday, Saturday football from now until the end of the season? Because that pretty much is what it is. We are going to have to manage the situation but we will give him every chance to be available after Saturday’s performance."
Garry Richards, dropped at the weekend, could be called upon to take King’s place.
The Gills head into their midweek clash having lost six of their last seven League 2 games.
"It’s a real big week coming up with three home games (in a row) against decent sides," said Hessenthaler "Saturday’s performance will give us a little bit more confidence and the fans that travelled will have seen that. They were right behind the team and I thank them for that."
The only player with a chance of coming into the squad from injury is midfielder Charlie Lee (knee).