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Defender Matt Fish has picked up an injury for Gillingham during a behind-closed-doors friendly against Reading.
The Gills fielded a mix of fringe first team players and youth team members in the game at Priestfield on Tuesday afternoon, which ended goalless.
Fish was joined in the team by Paulo Gazzaniga, Stefan Payne and Simon King.
A Gillingham XI also had a run-out on Saturday morning when they travelled to Crabble to face Nicky Forster's Dover Athletic team. The Gills won 3-2.
Boss Andy Hessenthaler knows he has to keep his reserve players on their toes.
He said: "It's important they keep getting minutes in their legs incase they get called upon. It is good that we are getting some football for them."
On Tuesday the Gills also fielded French trialist striker Cedrick Biemaraux. He played 90 minutes in the match.