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New signings Michael Richardson and Ryan Williams will taste action for the first time in a Gillingham shirt on Tuesday afternoon.
Dagenham & Redbridge are the visitors to town, just four days after the two teams met in League 1 action at Priestfield.
The game is being played behind closed doors and will give manager Martin Allen the chance to see what his two new arrivals from the Premier League can do.
Allen (pictured) is also set to hand many of the club’s young scholars a 20-minute spell at the end of the game.
The Gills boss said: “We are playing all our lads today who haven’t been playing regularly, to give them 70 minutes.
“The youth team, who have been working extremely hard on their fitness levels and their fast-track programme to be professionals, are going to be put on after 70 minutes to give them the experience to play, number one on our pitch and number two against good opposition.
“It is a kind of reward for all of those lads who have done fantastically well over the past six weeks and the work they have been putting in. We have another group (of youngsters) currently out on loan, about eight of them, and they are doing their thing at various clubs.”
Also set to play in the reserve game are the likes of keeper Tommy Forecast, defender Callum Davies, midfielders Steven Gregory and Bradley Dack, along with striker Adam Birchall.