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Trains across Kent will be tooting for carers at 8pm tonight - to coincide with the weekly Clap for Carers.
The special toot will spell out "N-H-S" in Morse code to give thanks to frontline workers amid the coronavirus pandemic.
It will compose of one long blast, followed by eight shorter toots.
Up to 50 Southeastern trains in depots and stations will be taking part across the county - and in London and East Sussex.
In Kent, residents living nearby Tonbridge station are set to hear the salute, as well as those living close to Slade Green, Ramsgate, Gillingham, St Leonards-On-Sea and Ashford depots.
David Statham, managing director for Southeastern, said: "We’ll be tooting for carers this evening to show our continued support to all those working for the NHS and in care roles.
"At Southeastern, we are immensely proud to play a part in keeping the country connected so that those keeping us all safe, can continue doing extraordinary work.
"Every day, our colleagues - from platform staff to train drivers - are inspired by the incredible work of those using our network. Our toot salute symbolises our immense thanks and praise."
People are encouraged to show their support if they hear the toot by tweeting #TootforCarers.