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Mark Stimson has hailed the League 1 clash against fellow strugglers Tranmere at Priestfield on Tuesday as "absolutely huge."
Only a second-half equaliser for Leyton Orient against Brighton stopped the Gills slipping into the bottom four on Saturday, while Oldham – 1-0 winners at Prenton Park – were the only side in the bottom six to claim maximum points.
Stimson said: "We are going into the game off a bad defeat, I will analyse what happened at Brentford with the rest of the coaching staff, and will pick a team who we think will get us a result.
"There might be a couple of changes possibly because I didn’t think one or two did themselves justice.
"If they aren’t big enough to hold their hand up and say ‘I am not on the button’, then I will make that decision, because we have got to win."
Stimson suffered a double blow on Friday when Rene Howe and Tristan Plummer were injured in training, continuing a jinx on loan players this season.
Stimson said: "Rene’s injury was bizarre, he went to have a shot, and somebody ran into him, hitting him around the hip area.
"It’s like a dead leg, but he went down straightaway and did well to get off the pitch. If you saw him then you would say he had no chance of being fit for Tuesday."