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Gillingham lost 1-0 at home to 10-man Oldham at Priestfield on Saturday afternoon.
Mike Petrasso's early second-half header settled the League 1 clash, while the visitors finished a man down after defender Genseric Kusunga clashed with Danny Kedwell in the closing stages.
Leading scorer Kedwell was among six changes from the FA Cup defeat at Brackley. He started up front alongside Cody McDonald having been expected to miss out with the shin injury suffered in the win at Sheffield United.
Oldham had the best of the first half with captain Korey Smith running the game from midfield. He saw plenty of the ball alongside James Wesolowski and Gills were unable to stop him pinging passes about all over the place.
It was Smith who went closest to opening the scoring as well. He was given space to turn and shoot on the edge of the area after 35 minutes but Stuart Nelson made a fine save low to his right.
Smith also sent a couple of efforts high and wide, while Danny Philliskirk's deflected strike was easily held.
Gillingham had little joy in attack, summed up when John Mousinho fell flat on his backside as he attempted a cross in front of the visiting Oldham fans.
The Latics dominated after the break and a goal looked a matter of time. It finally came on 57 minutes after more good work down the left.
Nelson did well to keep out Philliskirk's initial effort but Petrasso headed the loose ball over the Gills keeper.
Gillingham made a double substitution midway through the half, with Myles Weston replacing Danny Hollands while Craig Fagan came on for Chris Whelpdale.
Gills finally theratened after 70 minutes when McDonald latched on to Kedwell's flick and held off Jonathan Grounds but Mark Oxley made the save.
Kunsunga's dismissal, five minutes from time, gave Gills hope. They huffed and puffed but Oldham survived seven minutes of injury time to leapfrog Gills in League 1.
Gillingham: Nelson, Hewitt, Martin, Barrett (Dack 88mins), Legge, Hollands (Weston 65mins), Mousinho, Harriman, Kedwell, McDonald, Whelpdale (Fagan 65mins). Subs not used: Davies, Akinfenwa, Howard, Hessenthaler.