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Early indications are that Gillingham could be without new signing Lee Martin for the season.
The former Ipswich midfielder, signed this summer after his release from Millwall, went down with an ankle injury with 13 minutes left of the match at Leyton Orient.
It is understood the 29-year-old has dislocated and broken his left ankle.
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The severity of the injury led to Saturday’s pre-season match being abandoned on 77 minutes, with Orient leading 2-0.
Gills boss Justin Edinburgh, who has already been hit by a number of injuries in pre-season, said: “It’s not good news and from the physio assessing it, it looks like a broken and dislocated ankle. It is a real bad injury.”
Martin was taken to hospital straight away but Edinburgh is already braced for the worst.
The manager said: “We have most likely lost Lee for the season.”
Edinburgh’s preparations have already been rocked by Aaron Morris’ injury, which is still being assessed. A knee injury looks like keeping him out long-term.
Bradley Garmston is out for six to eight weeks with a dislocated knee cap and Scott Wagstaff is also out injured, with a strained calf.
Edinburgh said: “Our thoughts are with Lee. I know his mum and dad were at the game and we hope he makes a quick recovery.
“Unfortunately that is football and you know that it’s a contact sport and you can have injuries. We have had a spate of them but that’s what we have to put up with. We have to accept it and move on quickly.”