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Gillingham were dumped out of the Johnstone's Paint Trophy by League 2 Yeovil on penalties on Tuesday night.
Connor Roberts clinched a 5-4 shoot-out success for the Glovers after Gills' Rory Donnelly sent his kick into the stand.
Yeovil were unlucky not to win in normal time. They had the best of the game and led through Harry Cornick but Bradley Garmston equalised.
Gills made five changes to the side humbled by Stevenage in the FA Cup.
Bradley Dack and Josh Wright returned from suspension, Deji Oshilaja was back after two months out injured, Harry Lennon was available again and there was also a place for Garmston.
Among the casualties from Saturday’s starting XI were Jordan Houghton and Max Ehmer, who were left out of the squad, while Aaron Morris and Emmanuel Osadebe dropped to the bench. Jake Hessenthaler, a substitute on Saturday, was also left out of the 16.
Yeovil, with only a day’s rest after beating Maidstone in the FA Cup made a flying start.
Omar Sowunmi went close with a header and Shaun Jeffers hit the side-netting before a poor clearance from Stuart Nelson was returned by Ben Tozer whose 50-yard lob hit the bar.
Yeovil were taking Gills apart down the right but the opening goal came from the opposite flank with Harry Cornick catching out Oshilaja and then dumping John Egan on his backside before dinking the ball over Nelson for the 12th-minute opener.
Gillingham picked up marginally after going behind but it was an enthusiastic Glovers side who looked the better team for much of the half.
Gills threatened mdway through when Egan headed over Doug Loft’s cross.
But that was about it until a moment of quality from wing-back Garmston who cut inside from the left and bent an effort into the top corner two minutes before half-time.
Justin Edinburgh made a change at the break, Osadebe replacing Oshilaja as Gills switched from 3-5-2 to a midfield diamond.
Yeovil had a golden chance to go back in front when Jeffers found a way through the defence and fed Cornick who blazed over when well placed.
Matthew Dolan worked Nelson after neat work from Wes Fogden around the edge of the box as the visitors threatened again.
Yeovil had another big opportunity moving into the last 15 minutes.
Ryan Bird met a free-kick, sending the ball across the six-yard box to unmarked Alex Lacey who volleyed over when he should have scored.
Gills captain Loft thrashed an effort wide from 20 yards, substitute Ben Williamson had an effort cleared off the line and Dack worked Weale from the edge of the box as Gills finished strongly.
There was one last chance in injury time when Loft’s 30-yarder was well saved by Weale.
Dack, Egan, Loft and Williamson scored from the spot before Donnelly's miss handed Roberts the chance to send Yeovil through to the area semi-finals.
Gillingham: Nelson, Jackson, Garmston, Egan, Loft, McDonald (Williamson 79mins), Donnelly, Dack, Lennon, Wright (McGlashan 74mins), Oshilaja (Osadebe 46mins). Subs not used: G Morris, A Morris.
Yeovil: Weale, Tozer, Smith, Dolan, Jeffers (Allen 90mins), Sowunmi (Bird 66mins), Fogden, Lacey, Cornick, Roberts, Ward. Subs not used: Beck, Bassett, Needle.
Attendance: 1,832 (64 from Yeovil).