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Gillingham will give several senior players a run-out when they face Bournemouth in a development fixture at Priestfield.
The under-21 Premier League cup match takes place behind closed doors on Wednesday afternoon.
Gillingham are able to field four over-age players and boss Justin Edinburgh is due to give a start to Aaron Morris, who is now back from injury.
Defensive-midfielder Morris was on the bench for the first team at the weekend but faces a battle to get back into the team, due to the form of Josh Wright.
“Whoever is in that team has a hell of a job keeping their place and whoever is trying to get in has a harder one,” said the manager.
Strikers Rory Donnelly and Ben Williamson are also set to feature, as is keeper Glenn Morris.
Young left-back Bradley Garmston missed the cut for Saturday’s matchday squad and he will also play, as will Emmanuel Osadebe.
Those players will all be trying to impress and force their way into Edinburgh’s starting XI.
Edinburgh said: “Every player here feels they should play and rightly so. That is what we have worked towards but unfortunately only 11 can.”
Gillingham lost to AFC Wimbledon in the same competition last season.