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Gillingham will face League 2 Luton Town at home in the second round of the Johnstone’s Paint Trophy.
The Gills received a bye from round one and joined seven other teams in the draw for the south east section.
Matches will be played on the week commencing October 5.
Luton beat Leyton Orient in their first round match, with a last-minute goal from Stephen O’Donnell settling the tie.
The Gills reached the area final of last season’s competition, losing out to Bristol City.