Further strikes are to be held by railway workers in worsening disputes over pay, jobs and conditions, as passengers suffered travel misery on Wednesday because of another walkout.

Members of the drivers’ union Aslef and the Transport Salaried Staffs Association (TSSA) staged fresh action, leading to the cancellation of services across the country.

Picket lines were mounted outside railway stations as union leaders warned that the long-running dispute remains deadlocked.

Aslef announced that its members working on the Croydon Tramlink have rejected a pay offer and will strike on October 10 and 11.

The union’s London officer, Finn Brennan, said: “The management’s offer of 4.75% is far below the current rate of inflation and would mean our members face real-terms pay cuts.”

The workers have already staged two 48-hour strikes in the dispute.

Aslef general secretary Mick Whelan told the PA news agency that train drivers in rail operators in England face a third year without a pay rise, pointing out that deals have been achieved in Scotland and Wales.

He called on the Government to “lift the shackles” from train companies which he claimed are preventing them making a pay offer.

“The message I am receiving from my members is that they are in this for the long haul and, if anything, they want industrial action to be increased,” he said.

The train drivers’ strike will affect Avanti West Coast, Chiltern, CrossCountry, East Midlands Trains, Greater Anglia (including Stansted Express), Great Western, Hull Trains, LNER, London Overground, Northern Trains, Southeastern, Transpennine Express and West Midlands Trains.

Members of the TSSA will strike at CrossCountry and take other forms of industrial action at several other operators.

The TSSA is also planning industrial action on Thursday, Friday and Saturday, while members of the Rail, Maritime and Transport (RMT) union at more than a dozen train companies and Network Rail will strike on Saturday.

Rail companies not involved in the strike will be running trains, but these are expected to be busy.

Services are likely to be disrupted into the early morning of Thursday as workers return to duties.

The disruption will affect football fixtures and the final day of the Conservative Party conference in Birmingham.

Daniel Mann, director of industry operations at the Rail Delivery Group, said: “These strikes disrupt the travel plans of millions of passengers and undermine businesses who continue to struggle with rising costs and this continued action will only further damage the railway’s recovery.

“While some rail companies are not involved in the strike, services are likely to be busy, severely disrupted or in some cases not running, so passengers should check the latest travel advice before setting off.

“Passengers with advance, off-peak or anytime tickets affected by the strikes on 5 October can use their ticket on the day before the booked date, or up to and including 7 October.

“Passengers can also change their tickets to travel on an alternate date or get a refund if their train is cancelled or rescheduled.”

TSSA general secretary Manuel Cortes said: “We saw only last weekend that our strike action was rock-solid across the country. Our brilliant members remain determined to see their demands met in the shape of a just settlement.

“We do not strike lightly but are prepared to dig in as we seek a fair deal on pay, job security and conditions in the face of cuts, economic collapse and a cost-of-living crisis at the hands of this chaotic Tory Government.

“With more strikes and wider industrial action in the offing this week, it’s time for ministers to get wise and act now to deliver a fair deal which will halt a winter of rail misery across the network.”

A Department for Transport spokesman said: “It is incredibly disappointing that, for the second time in five days, we face disruption on our railways, with businesses once again being affected, thousands of people at risk of being unable to travel to work or school, and doctors’ appointments being missed.

“Our railway is in desperate need of modernisation but all more strikes will do is punish the very people unions claim to stand up for and push passengers further away.

“We urge union bosses to reconsider this divisive action and instead work with their employers, not against them, to agree a new way forward.”

A Department for Transport spokesperson said: “We’re committed to transforming accessibility across the UK rail network, and have dedicated £383 million for such improvements at rail stations which will deliver more than 100 accessible step-free routes and smaller-scale access enhancements at 124 stations.”