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The 2016 Ramsgate Sprint Revival has been cancelled.
The show, scheduled for August 13 and 14, will no longer go ahead after Thanet District Council failed to guarantee the group use of the port for a pit area.
Car imports and the possibility of additional port activity were the two major factors in the decision.
Ramsgate Sprint Revival was an event where classic motorbikes from the 1950s and 60s returned to Western Undercliff to demonstrate what they are made of over a quarter-mile sprint.
Organisers apologised for any inconvenience caused by their decision.
A spokesman said: “We have worked hard behind the scenes for a bigger and better event in 2016 but without guaranteed space for the motorcycles we cannot go ahead.
"Obviously the port must take priority and if TDC requires the space we have to accept that.”
Plans to run the show again in 2016 were postponed in November after the council confirmed land hire for Government Acre had been granted to a long standing event, Lark in the Park.
Leader of Thanet District Council Chris Wells set up a meeting in February between organisers of Lark in the Park and Ramsgate Sprint Revival, where agreement was reached for the two events to share Government Acre.
In the two weeks following the meeting it was confirmed that business at the Port of Ramsgate was increasing and it was not possible to guarantee space for a pit area for the sprint bikes and their support vehicles.
Initially the group had agreed with TDC that Military Road outside the port could be used for this purpose but subsequently sprint organisers were told that this could only be guaranteed at six weeks notice.